This detailed volume explores recent developments in microfluidics technologies for cancer diagnosis and monitoring. The book is divided into two sections that delve into techniques for liquid biopsy for cancer diagnosis and platforms for precision oncology or personalized medicine in order to create effective patient avatars for testing anti-cancer drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microfluidic Systems for Cancer Diagnosis serves as an ideal guide that will be helpful to either replicate the construction of microfluidic devices specifically developed for cancer diagnosis or to catalyze development of new and better cancer diagnostic devices.

1. Lateral Filter Array Microfluidic Devices for Detecting Circulating Tumor Cells

Kangfu Chen, Thomas J. George, and Z. Hugh Fan

2. Circulating Tumor Cell Cluster Sorting by Size and Asymmetry

Sam H. Au

3. Digital Microfluidics with an On-Chip Drug Dispenser for Single or Combinational Drug Screening

Caiwei Li, Jiao Zhai, and Yanwei Jia

4. Affinity-Based Microfluidics Combined with Atomic Force Microscopy for Isolation and Nanomechanical Characterization of Circulating Tumor Cells

Muhammedin Deliorman, Ayoub Glia, and Mohammad A. Qasaimeh

5. Capture and Selective Release of Viable Circulating Tumor Cells

Xilal Y. Rima, Jingjing Zhang, and Eduardo Reátegui

6. Single-Response Electronic Tongue and Machine Learning Enable the Multidetermination of Extracellular Vesicle Biomarkers for Cancer Diagnostics without Recognition Elements

Caroline Y. N. Nicoliche, Giulia S. da Silva, Letícia G. de Pontes, Gabriel R. Schleder, and Renato S. Lima

7. Functional Interrogation of Ca²⁺ Signals in Human Cancer Cells In Vitro and Ex Vivo by Fluorescent Microscopy and Molecular Tools

Chenyu Liang, Miao Huang, Mai Tanaka, Suzanne Lightsey, Madison Temples, Sharon E. Lepler, Peike Sheng, William P. Mann, Adrienne E. Widener, Dietmar W. Siemann, Blanka Sharma, Mingyi Xie, Yao Dai, Edward Phelps, Bo Zeng, and Xin Tang

8. Microfluidic Protocols for the Assessment of Anti-Cancer Therapies in 3D Tumor-Stromal Co-Cultures

Karla Paterson and Michele Zagnoni

9. A Microfluidic Approach for Enrichment and Single-Cell Characterization of Circulating Tumor Cells from Peripheral Blood

Payar Radfar, Lin Ding, Hamidreza Aboulkheyr Es, and Majid Ebrahimi Warkiani

10. Rapid On-Site Evaluation (ROSE): A Microfluidic Approach

Filipe Marques, Janosch Hauser, and Niclas Roxhed

11. Microfluidic Acoustic Method for High Yield Extraction of Cell-Free DNA in Low-Volume Plasma Samples

Alvaro J. Conde, Ieva Keraite, Nicholas R. Leslie, and Maïwenn Kersaudy-Kerhoas

12. Isolation of Extracellular Vesicles by a Microfluidic Platform to Diagnose and Monitor Pancreatic Cancer

María Sancho-Albero and Victor Sebastián

13. High-Throughput Separation and Enrichment of Rare Malignant Tumor Cells from Large-Volume Effusions by Inertial Microfluidics

Chen Ni, Zhixian Zhu, Zheng Zhou, and Nan Xiang

14. SAIF: Label-Free Separation of Circulating Tumor Cells Using a Self-Amplified Inertial Focusing Microfluidic Chip

Aynur Abdulla and Xianting Ding

15. Patient-Specific Microfluidic Cancer Spheroid Cultures for Testing Cancer Therapies

Daheui Choi, Alan M. Gonzalez-Suarez, Daniel D. Billadeau, Wen Wee Ma, Gulnaz Stybayeva, and Alexander Revzin

16. Isolation of Cancer Cells from Liquid Biopsies Using 3D-Printed Affinity Devices

Yijia Yang, Kitiara Griffin, Sarah Villareal, and Dimitri Pappas

17. A Microfluidic SERS Assay to Characterize the Phenotypic Heterogeneity in Cancer-Derived Small Extracellular Vesicles

Jing Wang, Matt Trau, and Alain Wuethrich

18. Cluster-Wells: A Technology for Routine and Rapid Isolation of Extremely Rare Circulating Tumor Cells Clusters from Unprocessed Whole Blood

Mert Boya and A. Fatih Sarioglu

19. Secretion Function Analysis of Ex Vivo Immune Cells in an Integrated Microfluidic Device

Roberto Rodriguez-Moncayo and Jose L. Garcia-Cordero

20. Dynamic Tumor Perfusion and Real-Time Monitoring in a Multiplexed 3D Printed Microdevice

Alex Markoski, Ian Y. Wong, and Jeffrey T. Borenstein

21. Capture and Release of Cancer Cells through Smart Bioelectronics

Janire Saez, Maite Garcia-Hernando, Achilleas Savva, Roisin M. Owens, Fernando Benito-Lopez, and Lourdes Basabe-Desmonts

22. Fabrication of Multilayer Microfluidic Arrays for Passive, Efficient DNA Trapping and Profiling

Christine M. O'Keefe and Tza-Huei Jeff Wang