With numerous methods available for the quantification of
nitric oxide (NO), this detailed book explores their advantages and
disadvantages in order to provide a foundation for further research in plant
nitric oxide. After providing a useful practical guide to choosing a technique
for measuring NO from plant materials, the book continues with chapters on chemiluminescence,
diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as
many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions
to their respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory methods, and tips on troubleshooting
and avoiding known pitfalls.
Practical and authoritative, Plant
Nitric Oxide: Methods and Protocolsserves as a valuable guide to all
researchers working and intending to work in the field of plant nitric oxide
research.

An Overview of Methods in Plant Nitric Oxide (NO) Research:
Why Do We Always Need to Use Multiple Methods?.- Chemiluminescence Detection of
Nitric Oxide from Roots, Leaves, and Root Mitochondria.- Nitric Oxide Measurement
from Purified Enzymes and Estimation of Scavenging Activity by Gas Phase
Chemlimunimescence Method.- Localization of Nitric Oxide in Wheat Roots by DAF
Fluorescence.- Nitric Oxide (NO) Measurements in Stomatal Guard Cells.- Methods
to Detect Nitric Oxide in Plants: Are DAFs Really Measuring NO?.- A Novel
Protocol for Detection of Nitric Oxide in Plants.- Detection of Nitric Oxide by
Electron Paramagnetic Resonance Spectroscopy: Spin-Trapping with
Iron-Dithiocarbamates.- Measurement of Nitric Oxide (NO) Generation Rate by
Chloroplasts Employing Electron Spin Resonance (ESR).- Laser-Based Methods for
Detection of Nitric Oxide in Plants.- Electrochemical Detection of Nitric Oxide
in Plant Cell Suspensions.- Quantification and Localization of S-Nitrosothiols
(SNOs) in Higher Plants.- Analysis of the Expression and Activity of Nitric
Oxide Synthase from Marine Photosynthetic Microorganisms.- Identification of
S-Nitrosothiols by the Sequential Cysteine Blocking Technique.- Detection of
S-Nitrosoglutathione Reductase Activity in Plants.- Detection of Peroxynitrite
in Plants Exposed to Bacterial Infection.