This detailed volume explores recent developments in microfluidics technologies for cancer diagnosis and monitoring. The book is divided into two sections that delve into techniques for liquid biopsy for cancer diagnosis and platforms for precision oncology or personalized medicine in order to create effective patient avatars for testing anti-cancer drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microfluidic Systems for Cancer Diagnosis serves as an ideal guide that will be helpful to either replicate the construction of microfluidic devices specifically developed for cancer diagnosis or to catalyze development of new and better cancer diagnostic devices.
1. Lateral Filter Array Microfluidic Devices for Detecting Circulating Tumor Cells
Kangfu Chen, Thomas J. George, and Z. Hugh Fan
2. Circulating Tumor Cell Cluster Sorting by Size and Asymmetry
Sam H. Au
3. Digital Microfluidics with an On-Chip Drug Dispenser for Single or Combinational Drug Screening
Caiwei Li, Jiao Zhai, and Yanwei Jia
4. Affinity-Based Microfluidics Combined with Atomic Force Microscopy for Isolation and Nanomechanical Characterization of Circulating Tumor Cells
Muhammedin Deliorman, Ayoub Glia, and Mohammad A. Qasaimeh
5. Capture and Selective Release of Viable Circulating Tumor Cells
Xilal Y. Rima, Jingjing Zhang, and Eduardo Reátegui
6. Single-Response Electronic Tongue and Machine Learning Enable the Multidetermination of Extracellular Vesicle Biomarkers for Cancer Diagnostics without Recognition Elements
Caroline Y. N. Nicoliche, Giulia S. da Silva, Letícia G. de Pontes, Gabriel R. Schleder, and Renato S. Lima
7. Functional Interrogation of Ca2+ Signals in Human Cancer Cells In Vitro and Ex Vivo by Fluorescent Microscopy and Molecular Tools
Chenyu Liang, Miao Huang, Mai Tanaka, Suzanne Lightsey, Madison Temples, Sharon E. Lepler, Peike Sheng, William P. Mann, Adrienne E. Widener, Dietmar W. Siemann, Blanka Sharma, Mingyi Xie, Yao Dai, Edward Phelps, Bo Zeng, and Xin Tang
8. Microfluidic Protocols for the Assessment of Anti-Cancer Therapies in 3D Tumor-Stromal Co-Cultures
Karla Paterson and Michele Zagnoni
9. A Microfluidic Approach for Enrichment and Single-Cell Characterization of Circulating Tumor Cells from Peripheral Blood
Payar Radfar, Lin Ding, Hamidreza Aboulkheyr Es, and Majid Ebrahimi Warkiani
10. Rapid On-Site Evaluation (ROSE): A Microfluidic Approach
Filipe Marques, Janosch Hauser, and Niclas Roxhed
11. Microfluidic Acoustic Method for High Yield Extraction of Cell-Free DNA in Low-Volume Plasma Samples
Alvaro J. Conde, Ieva Keraite, Nicholas R. Leslie, and Maïwenn Kersaudy-Kerhoas
12. Isolation of Extracellular Vesicles by a Microfluidic Platform to Diagnose and Monitor Pancreatic Cancer
María SanchöAlbero and Victor Sebastián
13. High-Throughput Separation and Enrichment of Rare Malignant Tumor Cells from Large-Volume Effusions by Inertial Microfluidics
Chen Ni, Zhixian Zhu, Zheng Zhou, and Nan Xiang
14. SAIF: Label-Free Separation of Circulating Tumor Cells Using a Self-Amplified Inertial Focusing Microfluidic Chip
Aynur Abdulla and Xianting Ding
15. Patient-Specific Microfluidic Cancer Spheroid Cultures for Testing Cancer Therapies
Daheui Choi, Alan M. Gonzalez-Suarez, Daniel D. Billadeau, Wen Wee Ma, Gulnaz Stybayeva, and Alexander Revzin
16. Isolation of Cancer Cells from Liquid Biopsies Using 3D-Printed Affinity Devices
Yijia Yang, Kitiara Griffin, Sarah Villareal, and Dimitri Pappas
17. A Microfluidic SERS Assay to Characterize the Phenotypic Heterogeneity in Cancer-Derived Small Extracellular Vesicles
Jing Wang, Matt Trau, and Alain Wuethrich
18. Cluster-Wells: A Technology for Routine and Rapid Isolation of Extremely Rare Circulating Tumor Cells Clusters from Unprocessed Whole Blood
Mert Boya and A. Fatih Sarioglu
19. Secretion Function Analysis of Ex Vivo Immune Cells in an Integrated Microfluidic Device
Roberto Rodriguez-Moncayo and Jose L. Garcia-Cordero
20. Dynamic Tumor Perfusion and Real-Time Monitoring in a Multiplexed 3D Printed Microdevice
Alex Markoski, Ian Y. Wong, and Jeffrey T. Borenstein
21. Capture and Release of Cancer Cells through Smart Bioelectronics
Janire Saez, Maite Garcia-Hernando, Achilleas Savva, Roisin M. Owens, Fernando Benito-Lopez, and Lourdes Basabe-Desmonts
22. Fabrication of Multilayer Microfluidic Arrays for Passive, Efficient DNA Trapping and Profiling
Christine M. O¿Keefe and Tza-Huei Jeff Wang