Preface...
Table of Contents...
Contributing Authors...
Part I Setting Up a Facility
1. Setting up Mass Cytometry in a Shared Resource Lab Environment
Sarah Warth and Désirée Kunkel
2. Acquisition, Processing, and Quality Control of Mass Cytometry Data
Brian H. Lee and Adeeb H. Rahman
Part II Panel Design and Reagent Preparation
3. Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating
Amelia Au-Yeung, Chikara Takahashi, William Mathews, and William E. O'Gorman
4. Method for Tagging Antibodies with Metals for Mass Cytometry Experiments
Stephen Gregory Chang and Cynthia J. Guidos
5. Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis
Felix J. Hartmann, Erin F. Simonds, Nora Vivanco, Trevor Bruce, Luciene Borges, Garry P. Nolan, Matthew H. Spitzer, and Sean C. Bendall
6. Titration of Mass Cytometry Reagents
Caryn van Vreden, Paula Niewold, Helen M. McGuire, and Barbara Fazekas de St Groth
7. Cell-Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry
Axel Ronald Schulz and Henrik E. Mei
Part III Sample Preparation
8. Automated Cell Processing for Mass Cytometry Experiments
Jaromir Mikes, Axel Olin, Tadepally Lakshmikanth, Yang Chen, and Petter Brodin
9. Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry
Lisa E. Wagar
Part IV Specific Applications
10. Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry
Diana Shinko, Thomas Ashhurst, Helen M. McGuire, and Kellie A. Charles
11. Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry
Yannick Simoni, Michael Fehlings, and Evan W. Newell
12. Analysis of the Murine Bone Marrow Haematopoietic System Using Mass and Flow Cytometry
Thomas M. Ashhurst, Darren A. Cox, Adrian L. Smith, and Nicholas J.C. King
13. Mass Cytometric Cell Cycle Analysis
Gregory K. Behbehani
14. Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry
Mikael Roussel, Todd Bartkowiak, and Jonathan M. Irish
15. Quantitative Measurement of Cell-nanoparticle Interactions Using Mass Cytometry
Andrew J. Mitchell, Angela Ivask, and Yi Ju
Part V Data Analysis
16. Data-Driven Flow Cytometry Analysis
Sherrie Wang and Ryan R. Brinkman
17. Analysis of Mass Cytometry Data
Christina B. Pedersen and Lars R. Olsen
18. Analysis of High Dimensional Phenotype Data Generated by Mass Cytometry or High Dimensional Flow Cytometry
Branko Cirovic, Natalie Katzmarski, and Andreas Schlitzer
19. Computational Analysis of High Dimensional Mass Cytometry Data from Clinical Tissue Samples
Sam Norton and Roslyn Kemp
20. Supervised Machine Learning with CITRUS for Single Cell Biomarker Discovery
Hannah G. Polikowsky and Katherine A. Drake